ImageSequences

Let’s import some packages

from neo.core import ImageSequence
from neo.core import RectangularRegionOfInterest, CircularRegionOfInterest, PolygonRegionOfInterest
import matplotlib.pyplot as plt
import quantities as pq

import random

Now we need to generate some data We will just make a nice box and then we can attach this ImageSequence to a variety of ROIs our ImageSequence will be 50 frames of 100x100 pixel images

l = []
for frame in range(50):
    l.append([])
    for y in range(100):
        l[frame].append([])
        for x in range(100):
            l[frame][y].append(random.randint(0, 50))

we then make our image sequence and pull out our results from the image_seq

image_seq = ImageSequence(l, sampling_rate=500 * pq.Hz, spatial_scale="m", units="V")

result = image_seq.signal_from_region(
    CircularRegionOfInterest(image_seq, 50, 50, 25),
    CircularRegionOfInterest(image_seq, 10, 10, 5),
    PolygonRegionOfInterest(image_seq, (50, 25), (50, 45), (14, 65), (90, 80)),
)

It is easy to plot our results using matplotlib

for i in range(len(result)):
    plt.figure()
    plt.plot(result[i].times, result[i])
    plt.xlabel("seconde")
    plt.ylabel("valeur")
plt.tight_layout()
plt.show()

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