Typical use cases¶
Recording multiple trials from multiple channels¶
In this example we suppose that we have recorded from an 8-channel probe, and
that we have recorded three trials/episodes. We therefore have a total of
8 x 3 = 24 signals, grouped into three
AnalogSignal objects, one per trial.
Our entire dataset is contained in a
Block, which in turn contains:
Segmentobjects, each representing data from a single trial,
ChannelIndex objects provide two different
ways to access the data, corresponding respectively, in this scenario, to access
by time and by space.
Segments do not always represent trials, they can be used for many purposes: segments could represent parallel recordings for different subjects, or different steps in a current clamp protocol.
Temporal (by segment)
In this case you want to go through your data in order, perhaps because you want to correlate the neural response with the stimulus that was delivered in each segment. In this example, we’re averaging over the channels.
import numpy as np from matplotlib import pyplot as plt for seg in block.segments: print("Analyzing segment %d" % seg.index) avg = np.mean(seg.analogsignals, axis=1) plt.figure() plt.plot(avg) plt.title("Peak response in segment %d: %f" % (seg.index, avg.max()))
Spatial (by channel)
In this case you want to go through your data by channel location and average over time. Perhaps you want to see which physical location produces the strongest response, and every stimulus was the same:
# We assume that our block has only 1 ChannelIndex chx = block.channelindexes: index = chx.channel_indexes siglist = [sig[:, index] for sig in chx.analogsignals] avg = np.mean(siglist, axis=0) plt.figure() for index, name in zip(chx.channel_indexes, chx.channel_names): plt.plot(avg[:, index]) plt.title("Average response on channels %s: %s' % (index, name)
Combining simultaneously the two approaches of descending the hierarchy temporally and spatially can be tricky. Here’s an example. Let’s say you saw something interesting on channel 5 on even numbered trials during the experiment and you want to follow up. What was the average response?
index = chx.channel_indexes avg = np.mean([seg.analogsignals[:, index] for seg in block.segments[::2]], axis=1) plt.plot(avg)
Recording spikes from multiple tetrodes¶
Here is a similar example in which we have recorded with two tetrodes and
extracted spikes from the extra-cellular signals. The spike times are contained
Again, our data set is contained in a
Block, which contains:
Segments(one per trial).
ChannelIndexes(one per tetrode), which contain:
Unitobjects (= 2 neurons) for the first
Unitsfor the second
In total we have 3 x 7 = 21
SpikeTrains in this
There are three ways to access the
In this example, each
Segment represents data from one trial, and we
want a PSTH for each trial from all units combined:
for seg in block.segments: print("Analyzing segment %d" % seg.index) stlist = [st - st.t_start for st in seg.spiketrains] plt.figure() count, bins = np.histogram(stlist) plt.bar(bins[:-1], count, width=bins - bins) plt.title("PSTH in segment %d" % seg.index)
Now we can calculate the PSTH averaged over trials for each unit, using the
for unit in block.list_units: stlist = [st - st.t_start for st in unit.spiketrains] plt.figure() count, bins = np.histogram(stlist) plt.bar(bins[:-1], count, width=bins - bins) plt.title("PSTH of unit %s" % unit.name)
Here we calculate a PSTH averaged over trials by channel location, blending all units:
for chx in block.channelindexes: stlist =  for unit in chx.units: stlist.extend([st - st.t_start for st in unit.spiketrains]) plt.figure() count, bins = np.histogram(stlist) plt.bar(bins[:-1], count, width=bins - bins) plt.title("PSTH blend of tetrode %s" % chx.name)
Spike sorting is the process of detecting and classifying high-frequency deflections (“spikes”) on a group of physically nearby recording channels.
For example, let’s say you have defined a ChannelIndex for a tetrode containing 4 separate channels. Here is an example showing (with fake data) how you could iterate over the contained signals and extract spike times. (Of course in reality you would use a more sophisticated algorithm.)
# generate some fake data seg = Segment() seg.analogsignals.append( AnalogSignal([[0.1, 0.1, 0.1, 0.1], [-2.0, -2.0, -2.0, -2.0], [0.1, 0.1, 0.1, 0.1], [-0.1, -0.1, -0.1, -0.1], [-0.1, -0.1, -0.1, -0.1], [-3.0, -3.0, -3.0, -3.0], [0.1, 0.1, 0.1, 0.1], [0.1, 0.1, 0.1, 0.1]], sampling_rate=1000*Hz, units='V')) chx = ChannelIndex(channel_indexes=[0, 1, 2, 3]) chx.analogsignals.append(seg.analogsignals) # extract spike trains from each channel st_list =  for signal in chx.analogsignals: # use a simple threshhold detector spike_mask = np.where(np.min(signal.magnitude, axis=1) < -1.0) # create a spike train spike_times = signal.times[spike_mask] st = neo.SpikeTrain(spike_times, t_start=signal.t_start, t_stop=signal.t_stop) # remember the spike waveforms wf_list =  for spike_idx in np.nonzero(spike_mask): wf_list.append(signal[spike_idx-1:spike_idx+2, :]) st.waveforms = np.array(wf_list) st_list.append(st)
At this point, we have a list of spiketrain objects. We could simply create a single Unit object, assign all spike trains to it, and then assign the Unit to the group on which we detected it.
u = Unit() u.spiketrains = st_list chx.units.append(u)
Now the recording channel group (tetrode) contains a list of analogsignals, and a single Unit object containing all of the detected spiketrains from those signals.
Further processing could assign each of the detected spikes to an independent source, a putative single neuron. (This processing is outside the scope of Neo. There are many open-source toolboxes to do it, for instance our sister project OpenElectrophy.)
In that case we would create a separate Unit for each cluster, assign its spiketrains to it, and then store all the units in the original recording channel group.